| 摘要 |
PROBLEM TO BE SOLVED: To conveniently and quickly measure lipoxygenase activity in grains e.g. for beer-brewing raw material which participates in oxidation reaction of lipid by reacting a grain extract with a substrate solution and then adding FOX(Ferrous oxidation/xylenol orange method) reagent to the reaction product, followed by measuring absorbance. SOLUTION: When reacting a grain extract e.g. comprising an extract of barley or malt with a substrate solution containing a free fatty acid (or its ester) such as linoleic acid or phosphatidylcholine dilinoleyl, dilinolein or trilinolein as a substrate, by terminating the reaction through the addition of an organic solvent such as methanol dissolving the free fatty acid or an organic solvent such as 1-butanol extracting neutral fat, or the like, recovering a fraction containing the reaction product, adding FOX reagent to the fraction and by measuring absorbance, lipoxygenase activity in grain which participates in oxidation reaction of lipid is measured conveniently in a short time. |
