发明名称 |
TISSUE-LIKE ORGANIZATION OF CELLS AND MACROSCOPIC TISSUE-LIKE CONSTRUCTS, GENERATED BY MACROMASS CULTURE OF CELLS, AND THE METHOD OF MACROMASS CULTURE |
摘要 |
<p>Three-dimensional tissue-like organization of cells by high cell-seeding-density culture termed as macromass culture is described. By macromass culture, cells can be made to organize themselves into a tissue-like form without the aid of a scaffold and three-dimensional macroscopic tissue-like constructs can be made wholly from cells. Tissue-like organization and macroscopic tissue-like constructs can be generated from fibroblastic cells of mesenchymal origin (at least), which can be either differentiated cells or multipotent adult stem cells. In this work, tissue-like organization and macroscopic tissue-like constructs have been generated from dermal fibroblasts, adipose stromal cells-derived osteogenic cells, chondrocytes, and from osteoblasts. The factor causing macroscopic tissue formation is large scale culture at high cell seeding density per unit area or three-dimensional space, that is, macromass culture done on a large scale. No scaffold or extraneous matrix is used for tissue generation, the tissues are of completely cellular origin. No other agents (except high cell-seeding-density) that aid in tissue formation such as tissue-inducing chemicals, tissue-inducing growth factors, substratum with special properties, rotational culture, etc, are employed for tissue formation. These tissue-like masses have the potential for use as tissue replacements in the human body. Tissue-like organization by high cell-seeding-density macromass culture can also be generated at the microscopic level.</p> |
申请公布号 |
WO2005095585(A1) |
申请公布日期 |
2005.10.13 |
申请号 |
WO2004IN00086 |
申请日期 |
2004.03.31 |
申请人 |
RELIANCE LIFE SCIENCES PVT LTD;DESHPANDE, MANISHA, SHARADCHANDRA;MOJAMDAR, MANOJ, VINOY |
发明人 |
DESHPANDE, MANISHA, SHARADCHANDRA;MOJAMDAR, MANOJ, VINOY |
分类号 |
A61K35/12;C12N5/00;C12N5/06;C12N5/08;(IPC1-7):C12N5/06 |
主分类号 |
A61K35/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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