METHODS FOR THE REDUCTION OF DISULFIDE BONDS

摘要

This invention provides a method for reducing disulfide bonds in a chemical agent, the method may include, inter alia, the step of contacting the chemical agent with 1,4-dimercapto-2,3-dihydroxybutane, wherein the 1,4-dimercapto-2,3­dihydroxybutane is prepared by a non-stereoselective reaction. This invention further provides a method for protecting a protein against oxidation, the method may include, inter alia, the step of contacting the protein with 1,4-dimercapto-2,3­dihydroxybutane, wherein the 1,4-dimercapto-2,3-dihydroxybutane is prepared by a non-stereoselective reaction. This invention further provides a method for reducing a disulfide bond in a. chemical agent, the method may include, inter alia, the step of contacting the chemical agent with a compound represented by the structure of formula (I) wherein R1, R2 and R3 are the same or different, independently of each other represent H, an unsubstituted or substituted linear, branched or cyclic hydroxyalkyl, an unsubstituted or substituted linear, branched or cyclic hydroxyalkenyl or an unsubstituted or substituted hydroxyaryl, an unsubstituted or substituted linear, branched or cyclic alkoxyalkyl ether, an unsubstituted or substituted linear, branched or cyclic alkoxy alkoxyalkyl ether, an unsubstituted or substituted linear, branched or cyclic alkylthioalkyl ether. This invention further provides a method for protecting a protein against oxidation, the method may include, inter alia, the step of contacting the protein with a compound represented by the structure of formula (I) wherein R1, R2 and R3 are the same or different, independently of each other represent H, an unsubstituted or substituted linear, branched or cyclic hydroxyalkenyl, an unsubstituted or substituted linear, branched or cyclic hydroxyalkenyl or an unsubstituted hydroxyaryl, an unsubstituted or subsituted linear, branched or cyclic alkoxyalkyl ether, an unsubstituted or substituted linear, branched or cyclic alkoxy alkoxyalkyl ether, an unsubstituted or substituted linear, branched or cyclic alkylthioalkyl ether.