System for inducible, local and reversible gene silencing using RNA interference

摘要

The present invention relates to a combination of expression cassettes for interfering with the expression of at least one gene in a cell or a vertebrate comprising (a) an expression cassette comprising a first promoter and operatively linked thereto a polynucleotide encoding an inducible transactivator (ITA); (b) one or more expression cassettes comprising polynucleotide(s) encoding one or more trans-acting factors (TAFs) heterologous to said cell or vertebrate and necessary and sufficient to functionally interact with a pol I promoter wherein expression of at least one of said TAFs is directed by a second promoter inducible by the transactivator; and (c) at least one expression cassette comprising at least one pol I promoter and operatively linked thereto one or more polynucleotides giving rise upon transcription and optionally post-transcriptional modifications RNA molecules capable of interfering with gene expression.