发明名称 |
PEPTIDE ANALYSIS USING A SOLID SUPPORT |
摘要 |
The present invention relates to a method of identifying a polypeptide, whic h method comprises the steps of (a) derivatization of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, wi th at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern, and (c) interpreting the fragmentation pattern obtained, wherein the peptide or polypeptide is immobilized to a solid suppo rt at least during step(a). Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique.
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申请公布号 |
CA2448534(A1) |
申请公布日期 |
2002.11.28 |
申请号 |
CA20022448534 |
申请日期 |
2002.05.20 |
申请人 |
AMERSHAM BIOSCIENCES AB;THE PROCTER AND GAMBLE COMPANY |
发明人 |
LIMINGA, MARIA;KEOUGH, THOMAS W.;YOUNGQUIST, ROBERT S.;VAUGHN, HAROLD L.;YELM, KEN E.;PALMGREN, RONNIE;MALOISEL, JEAN-LUC;BHIKHABHAI, RAMAGAURI |
分类号 |
G01N27/62;G01N33/58;G01N33/68;(IPC1-7):G01N33/58;C12Q1/37 |
主分类号 |
G01N27/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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