ENHANCEMENT OF FREEZING TOLERANCE IN TRANSGENIC PLANTS

摘要

The present invention relates to the transformation of a plant cell with a nucleic acid encoding a cold tolerance polypeptide from the Wcor410 family, possibly combined with a period of low temperature exposure, for increasing its viability, for improving its low temperature, freezing or cross-stress tolerance as well as its membrane stability, fruit and leafy vegetable stability, seed stability and shelf life at low temperature and for improving plant productivity, resistance to osmotic or pathogen stress. More generally, it relates to the transformation of any cell with a nucleic acid encoding a cold tolerance polypeptide from the Wcor410 family for improving its metabolic stability and activity at low temperature or for its cryoprotection, as well as the protection of food or drug by the addition of a cold tolerance polypeptide from the Wcor410 family. A method for plant transformation based on high kanamycin selection pressure is also claimed.