| 主权项 |
1. A method for identifying one or more proteins in a sample, comprising:
A. introducing the protein or proteins into a microreactor chamber or chambers contained in an array, at a concentration that results in a single protein molecule in the microreactor chamber, and wherein the microreactor chamber contains an endopeptidase, and an amine-reactive surface to which the microreactor chamber is sealed; B. allowing the endopeptidase to digest the protein or proteins into peptides; C. allowing the peptides to react with the amine-reactive surface, resulting in the peptides attaching in a cluster on the amine-reactive surface, wherein the cluster is associated with the single protein molecule and if more than one protein has been introduced into the microreactor chambers, an arrangement of the clusters on the amine-reactive surface of the array forms a recognizable pattern; D. labeling the peptides at specific amino acid residues, such that the specific amino acid residues can be detected when imaged; E. imaging the peptides to detect the labeled amino acids and generate amino acid sequences; F. digesting the peptides with enzymes or chemicals that digest the peptides at specific amino acid residues and imaging the resulting peptides to generate additional amino acid sequences; G. comparing the generated amino acid sequences of the peptides from the single protein molecule or molecules with known amino acid sequences of proteins; and H. identifying the protein or proteins from the comparison in step (G). |
