| 摘要 |
The invention tackles the problem of oligonucleotides forming complexes between or within themselves. Firstly, the sequences of oligonucleotides are tested for potential secondary structures. Then, when designing the sequences of the oligonucleotides, the nucleotides responsible for forming hydrogen bonds in the secondary structures, are swapped. They are replaced by universal or specifically binding nucleotides. In the current proof of principle, pairs of 2-amino-dA and 2-thio-dT are used to demonstrate that the balance between probe-probe and probe-target hybridization becomes shifted in the more favoured direction. This results in stronger signals and lesser false signals. |
