METHOD OF ASSAY OF CHEMOKINE RECEPTOR CCR2 GENE ALLELES AT POLYMORPHOUS SITE V64I

摘要

molecular biology, medicine. SUBSTANCE: to assay chemokine receptor gene polymorphism DNA is isolated from a sample to be studied followed by DNA amplification by polymerase chain reaction method using allele-specific primers. To identify the wide-spread allele CCR2 64V the direct primer 5'- TGCGCATTTATTAAGATGAGGTC-3' is used; to identify the rare allele CCR2 64I the direct primer 5'-TGCGCAGTTTATTAAGATGAGGCT-3' is used; in both cases the sequence 5'- GTGTGGTCATCATTTTGTTCTTTG-3; is used as the reverse primes. The presence of gene alleles CCR2 in sample is proved by appearance amplified DNA fragment of size 296 nucleotide pairs on gele after electrophoresis carrying out. The detection of indicated fragment after DNA amplification with both primer pairs indicates the presence of both alleles while amplification of fragment with one pair of primers only indicates the presence of one corresponding allele of CCR2. Invention provides possibility to perform highly precise, simple and cheap analysis of chemokine receptor gene variants useful for total application in clinical laboratory. EFFECT: improved method of gene allele assay. 2 dwg, 1 ex