METHOD OF DIAGNOSING RHEUMATOID ARTHRITIS

摘要

This invention provides a method for diagnosing rheumatoid arthritis whic h comprises digesting human genomic DNA with a restriction enzyme EcoRI and detecting an about 7 kb DNA fragment which hybridizes with a probe having th e base sequence of the position 159-424 in SEQ ID NO: 1, or which comprises digesting human genomic DNA with a restriction enzyme Dral and detecting an about 1.5 kb DNA fragment which hybrizes with a probe having the base sequence of the position 159-424 in SEQ ID NO: 1. The invention also provide s a method for diagnosing rheumatoid arthritis which comprises stimulating the peripheral blood mononuclear cells in a subject with phorbol 12-myristate 13-acetate (PMA) and/or phytohemagglutinin (PHA), and comparing the amount o f expression product in the peripheral blood mononuclear cells with the amount of the same product in the peripheral blood mononuclear cells in a healthy pers on stimulated by PMA and/or PHA, wherein the expression product is expressed fr om the base sequence of at the least 689-760 position in SEQ ID NO: 1.