| 摘要 |
PROBLEM TO BE SOLVED: To screen anti-white hair agents by utilizing a migration phenomenon of melanocyte to a hair papilla by bringing a specific melanocyte to a culture supernatant liquid of hair papilla and observing the degree of migration of melanocyte to the cultured supernatant liquid. SOLUTION: (A) Melanocyte is cultured in the presence of a test article. The cultured melanocyte is brought into contact with (B) a culture supernatant liquid of a hair papilla cell. Whether the component A is migrated to the component B is observed or the degree of the migration is observed to screen anti- white hair agents. For example, the component B is obtained by vertically cutting a scalp piece excised from a normal Japanese head orthopedically right under corium, extracting hair follicle, collecting a hair papilla from the isolated human hair papilla, leaving the hair papilla to stand in a culture laboratory dish, subculturing only a cell outgrowing from the implanted hair papilla in a culture flask and collecting a cultured supernatant liquid in subculture of three to seven times.
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