发明名称 |
AVIAN PLURIPOTENT EMBRYONIC GERM CELL LINE |
摘要 |
Disclosed in this invention is a process for preparing an established avian embryonic germ cell line comprising the steps of: (a) culturing primordial germ cells (PGCs) isolated from an avian embryonic gonad in a medium supplemented with a cell growth factor and a differentiation inhibitory fact or to obtain EG cell colonies; (b) culturing the EG cells in the same medium as in step (a) by employing a feeder layer until the EG cells are colonized; an d (c) recovering and subculturing the EG cells in the same medium as in step ( a) to establish the EG cell line. |
申请公布号 |
CA2359775(A1) |
申请公布日期 |
2000.08.17 |
申请号 |
CA20002359775 |
申请日期 |
2000.02.11 |
申请人 |
HAN, JAE YONG;HANMI PHARM. CO., LTD. |
发明人 |
PARK, TAE SUB;HAN, JAE YONG |
分类号 |
C12N15/09;C12N5/073;C12N5/10;C12Q1/02;(IPC1-7):C12N5/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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