| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new nucleic acid fragment comprising a gyrB gene having a specific base sequence, and capable of rapidly, readily and specifically detecting and counting specified microorganisms capable of degrading trichloroethylene or the like and useful for purification of polluted environment, in good sensitivity. SOLUTION: This fragment is the new nucleic acid fragment comprising a gyrB gene having a base sequence selected from formulas I to III, and useful for rapidly, readily and specifically detecting and counting microorganisms such as Vibrio sp. KB1 strain (FERM P-14643), JM1 strain (FERM BP-5352) and Pseudomonas cepacia KK01 strain (FERM BP-4235), capable of degrading trichloroethylene or the like and useful for purification of polluted environment in good sensitivity. The nucleic acid fragment is obtained by comparing base sequences of gyrB genes of various known Gram-negative bacteria and amplifying each nucleic acid obtained from the before microorganisms by using a primer set from a common sequence region obtained by the comparison, by a PCR. |
