Biochemical and immunological applications of a rat glutation transferase-histidine decarboxylase fusion protein

摘要

Biochemical and immunological applications of a rat glutation transferase-histidine decarboxylase fusion protein. By reverse transcription (RT) and chain amplification (PCR) a rat histidine decarboxylase cDNA with residues from 1 to 512 (rHDC1/512) was obtained and this was used to construct a recombinant plasmid capable of expressing the fusion of glutation transferase (GST) with rHDC1/512. Purification of polypeptides with HDC activity is considerably simplified and cheapened through the use of this fusion (GST-rHDC1/512) in comparison with the acquisition of HDC protein from natural sources. The fusion protein can be used to search for and characterise antihistamine drugs which have histidine decarboxylase activity as a target and for the investigation of post- translational affinity, degradation and modification of the HDC polypeptide. Starting with the GST-rHDC1/512 fusion protein polyclonal antibodies capable of recognising the original rat HDC antigen were obtained, and these are of interest in the development of purification and diagnostic methods for the protein.