| 摘要 |
PROBLEM TO BE SOLVED: To obtain a polynucleotide encoding a new collectin which exhibits antibacterial, antiviral activity or the like in vivo in humans and is useful as a physiologically active medicinal substance. SOLUTION: This polynucleotide contains a base sequence encoding a protein constituted of an amino acid sequence of the formula. This polynucleotide is obtained by the following procedure: regions with higher preservability among known collections are retrieved, an EST database is retrieved to afford data (registry number: R 29493) containing unknown base sequences; from a clone as the base of the above data, a probe for screening use is prepared; a human liver-derived cDNA library subjected to titration is screened with the probe, a positive clone is subjected to secondary screening in a similar way, the base sequence of the whole region of the plasmid of a transformant afforded by transformation with a DNA fragment based on the resulting positive clone is determined, thus obtaining the aimed cDNA clone encoding the amino acid sequence of the formula. |
