| 摘要 |
PCT No. PCT/US94/09120 Sec. 371 Date Oct. 11, 1994 Sec. 102(e) Date Oct. 11, 1994 PCT Filed Aug. 15, 1994 PCT Pub. No. WO95/06064 PCT Pub. Date Mar. 2, 1995A composition is provided comprising about 0.1 to 15 mg/ml of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 mu M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.
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