DETERMINATION OF LDL CHOLESTEROL

摘要

PROBLEM TO BE SOLVED: To easily determine the subject compound without using pretreatment of a specimen by incorporating serum with a specific surfactant and an enzymatic reagent for the determination of cholesterol, reacting cholesterols other than LDL cholesterol and determining the amount of reacted cholesterol. SOLUTION: Low-density lipoprotein(LDL) cholesterol is easily determined by incorporating serum with a surfactant such as a polyoxyalkylene phenyl ether or a polyoxyethylene alkylene tribenzylphenyl ether, a substance forming a polyanion or a bivalent metal ion exhibiting a bonding affinity to very lowdensity lipoprotein(VLDL) stronger than the affinity to lowdensity lipoprotein(LDL) and an enzymatic reagent for the determination of cholesterol, preferentially reacting the cholesterols in the high-density lipoprotein(HDL) and in the very low-density lipoprotein(VLDL) among the lipoproteins and determining the reaction amount of cholesterol after the preferential reaction.