发明名称 |
IN VITRO FLUORESCENCE POLARIZATION ASSAY |
摘要 |
An in vitro assay method permits the identification of a test substance which inhibits the mutual association of a pair of proteins. The method includes the steps of providing a pair of proteins capable of mutual association, one of said proteins bearing a covalently linked fluorophore; preparing a mixture containing the two proteins and at least one test substance; irradiating the mixture with polarized light of a suitable wavelength permitting excitation of the fluorophore as indicated by emission of polarized light; measuring the degree of polarization of the emission, and determining the effect of the presence or concentration of the test substance in decreasing the observed emission polarization of a mixture of the two proteins alone. Inhibitory activity of in the test substance correlates with decreased depolarization values.
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申请公布号 |
CA2250067(A1) |
申请公布日期 |
1997.10.23 |
申请号 |
CA19972250067 |
申请日期 |
1997.04.18 |
申请人 |
ARIAD PHARMACEUTICALS, INC. |
发明人 |
LYNCH, BERKLEY A.;MACNEIL, IAN A.;ZOLLER, MARK J. |
分类号 |
G01N21/64;G01N33/542;(IPC1-7):G01N33/542;G01N33/566 |
主分类号 |
G01N21/64 |
代理机构 |
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主权项 |
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地址 |
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