| 摘要 |
The invention relates to a process for detecting and quantifying nucleic acid (=NA) molecules within a population (I) of NA molecules of identical, similar or different sequence, in which: a) heteroduplexes are formed in which nucleotides which are unpaired or, according to Watson and Crick, incorrectly paired are chemically reactive with a carbon di-imide compound; b) the single or double-stranded ribonucleic acid (=RNA) molecules or single-stranded deoxyribonucleic acid (=DNA) molecules contained in population (I) are transferred into a population (II) of double-stranded DNA molecules; c) the population (II) of double-stranded DNA molecules are subjected to denaturation and subsequent renaturation, to produce a mixture of homo and heteroduplex DNA molecules. To make it possible to quantify the NA molecules detected, it is proposed that a purification stage take place between steps a) and b) and between steps b) and c). |
