发明名称 |
Efficient gene probe conjugations by an unconventional mixed anhydride method |
摘要 |
A novel method for conjugating haptens, ligands or luminescent labels to polynucleotides has been developed. This method involves the formation of a mixed anhydride, followed by reaction of the mixed anhydride intermediate with a nucleophilic group-containing polynucleotide in a DMF/water solvent system. The resulting compound finds usefulness especially in the gene probe area.
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申请公布号 |
US5622825(A) |
申请公布日期 |
1997.04.22 |
申请号 |
US19940358963 |
申请日期 |
1994.12.19 |
申请人 |
CIBA CORNING DIAGNOSTICS CORP. |
发明人 |
LAW, SAY-JONG;LUKINSKY, HANA |
分类号 |
C07H21/04;C07H21/00;C12N15/00;C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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