DISPOSABLE REAGENT STORAGE AND DELIVERY CARTRIDGE

摘要

A disposable cartridge (1) for storage and delivery of reagents for synthesizing and labeling of synthetic oligonucleotides comprises an annular casing (2), an enclosed area (4) defined by the interior surface of the casing and a non-swellable matrix (5) which is inert to organic solvents is positioned within the enclosed area (4). Adsorbed onto the surface of the matrix (5) is a reagent to be coupled to an immobilized oligonucleotide precursor. The cartridges (1) can be packaged in such a way as to insure the long-term stability of the reagents contained therein. The cartridge (1) is readily insertable into the reagent delivery line of an automated synthesizer of oligonucleotides and also is useful for the production of labeled oligonucleotides by manual coupling. The cartridges (1) of the invention are disposable after a single use. Substantial economies in the synthesis of labeled synthetic oligonucleotides are achieved by use of the cartridge (1).