Gentherapeutisches Verfahren unter Verwendung von DNA-Vektoren ohne Selektionsmarker-Gen

摘要

The use of a circular vector DNA to manufacture a drug to treat mammals or humans by gene therapy, wherein the vector DNA contains a selection marker gene and a DNA sequence that is heterologous for the vector and that causes a modulation, correction or activation of the expression of an endogenous gene or the expression of a gene introduced into the cells of the mammal or human by the vector DNA, is characterized in that the vector nucleic acid (a) is amplified under selection pressure and is split in such a way that said selection marker gene and said heterologous DNA are present on separate DNA fragments, (b) the DNA fragment that contains said heterologous DNA or both fragments are recircularized into vectors, (c) said DNA fragments are separated before or after the recircularizing, (d) the recircularized DNA fragment which contains said heterologous DNA is isolated and (e) the recircularized DNA fragment thus obtained is used to manufacture the drug.