发明名称 |
Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease |
摘要 |
The present inventor have identified of the recognition and cleavage domains of the FokI restriction endonuclease. Accordingly, the present invention relates to DNA segments encoding the recognition and cleavage domains of the FokI restriction endonuclease, respectively. The 41 kDa N-terminal fragment constitutes the FokI recognition domain while the 25 kDa C-terminal fragment constitutes the FokI cleavage nuclease domain. The present invention also relates to hybrid restriction enzymes comprising the nuclease domain of the FokI restriction endonuclease linked to a recognition domain of another enzyme.
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申请公布号 |
US5356802(A) |
申请公布日期 |
1994.10.18 |
申请号 |
US19920862831 |
申请日期 |
1992.04.03 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
CHANDRASEGARAN, SRINIVASAN |
分类号 |
C12N9/22;C12N15/55;C12Q1/68;(IPC1-7):C12N9/22;C12N15/62 |
主分类号 |
C12N9/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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