| 摘要 |
<p>Disclosed is an apparatus and method for the rapid flowthrough assay of an analyte. A multiplicity of binding sites which bind selectively to an analyte are immobilized on a rigid matrix, which defines a flowpath. A liquid sample containing the analyte is delivered convectively to a hydraulically full volume through the matrix to permit formation of a binding site: analyte complex. A detectable tagging reagent capable of binding with the binding site or the complex is then delivered convectively to the matrix and the amount of tagging reagent which is bound is detected to provide an assay of the concentration or presence of the analyte.</p> |
