发明名称 |
METHOD FOR STABILIZING NUCLEIC ACID |
摘要 |
<p>Methods of immobilizing nucleic acid on a solid surface for us in nucleic acid hybridization assays is disclosed. The methods of the invention comprise reacting a modified nucleic acid strand comprising a variable portion and an anchor portion wherein the variable portion comprises a nucleotide sequence having a selected base sequence and the anchor portion comprises at least one nucleotide base modified with a primary amine function or nucleotide base equivalent having a primary amine function and reacting the modified nucleic acid strand with a free aldehyde group of the solid surface in the presence of a reducing agent to form complexes of the modified nucleic acid strand and at least a portion of the free aldehyde groups on the solid surface.</p> |
申请公布号 |
GR900100828(A) |
申请公布日期 |
1992.04.17 |
申请号 |
GR19900100828 |
申请日期 |
1990.11.28 |
申请人 |
ORTHO DIAGNOSTIC SYSTEMS INC. |
发明人 |
BAHL CHANDER;LANG RHONDA;MENDOZA LEO |
分类号 |
C07H21/00;C12Q1/68;G01N33/543;(IPC1-7):G01N33/543 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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