| 摘要 |
NEW MATERIAL:A specific antigen protein from Bacteroides ginvalis, having the amino acid sequence given in the formula. USE:An immunological diagnostic for adult periodontitis. PREPARATION:For example, the DNA containing the specific antigen gene originating from Bacteroides ginvalis coding the amino acid sequence given in the formula is bonded to an appropriate vector to prepare a recombined plasmid. The host such as Escherichia coli is transformed by plasmid insertion, inoculated in a culture medium and subjected to shake culture. The culture mixture is centrifuged to collect the cell bodies, which are dispersed in a buffer and crushed by means of ultrasonic vibration. The product is centrifuged to collect the supernatant, the supernatant is saturated with ammonium sulfate and gel-filtered to obtain the subject antigen protein with the amino acid sequence given in the formula. |
