| 摘要 |
PURPOSE:To permit measurement of the number of bacteria in real time by projecting UV rays to sample air during sampling at a prescribed suction rate and measuring the number of pulses, pulse crest value and pulse width of the fluorescent spectra emitted from the bacteria. CONSTITUTION:The light from an ultra-high pressure mercury lamp 1 is focused through a condenser lens 2 and an excitation filter 3 to a UV ray excitation part 5 in a sample air introducing pipeline 4. On the other hand, a suction pump 9 is connected through a flow meter 7 and a needle valve 8 to a terminal 4b of the pipeline 4 and the UV rays are projected to this air to cause the bacteria in the air sample to emit excited fluorescence. The fluorescent spectra from the bacteria are condensed by a condenser lens 10 and are introduced to a spectroscope 11. The spectra are further converted to an electric signal by a photomultiplier 12. The electric signal is inputted through an amplifier 13 to a wave analyzer 14. The number of pulses, pulse crest value, and pulse width are simultaneously measured in the analyzer 14 and the bacteria in the sample air are measured in real time from the measured value.
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