CHEMICAL LUMINESCENCE AMPLIFICATION SUBSTRATE SYSTEM FOR IMMUNO CHEMISTRY

摘要

A system for the detection of a biological analyte of interest is disclosed which comprises an encapsulated fluorescer material which has been conjugated to an immunological specie specific to the biological analyte of interest, a means of disrupting the capsule containing the fluorescer and an energy source other than electro-magnetic radiation which is capable of activating the fluorescer. A method for the qualitative and/or quantitative detection of a biological of interest is disclosed, which comprises: (a) labeling an immunological specie specific to the analyte of interest with an encapsulated fluorescer material which is biologically compatible with such specie; (b) contacting the encapsulated fluorescer labeled specie and the biological of interest to form an encapsulated fluorescer labeled specie/biological complex; (c) separating. the encapsulated fluorescer labeled specie/biological compex; (d) disrupting the capsule containing the fluorescer label thus freeing it to solution; (e) contacting the freed fluorescer with an energy source other than electro-magnetic radiation which is capable of activating the fluorescer label; and (f) determining the presence of and/or measuring the quantity of chemiluminescent light emitted.