| 摘要 |
The protein MP1 has the following parameters: a) an electrophoretic mobility in the range between alpha 2 and beta 1 globulins; b) an isoelectric point of 4.75 +/- 0.15; c) a sedimentation coefficient S20,w of 6.65 +/- 0.15 S; d) a molecular weight, determined in a polyacrylamide gel containing sodium dodecyl sulphate (SDS), of 180,000 +/- 20,000, the molecules of MP1 being composed of apparently identical subunits which have a molecular weight of 90,000 +/- 10,000 and are held together non-covalently; e) an extinction coefficient Em (280 nm) of 8.2 +/- 0.4; f) a carbohydrate content of 9.6 +/- 2.5 % (mannose 2.2 +/- 0.5 %, galactose 1.7 +/- 0.4 %, fucose 0.4 +/- 0.2 %, N-acetylglucosamine 3.2 +/- 0.8 %, N-acetylneuraminic acid 2.1 +/- 0.6 %) and g) a defined amino-acid composition. MP1 can be used to prepare antisera which can be used to detect and determine MP1. In addition, antibodies against MP1 can be employed for the radioimmunological localisation and immunotherapeutic treatment of tumours which produce MP1. |
