Prepn. of cell-free penicillin-acylase by fermenting a strain of E. coli which produces this enzyme. The intracellular enzyme is separated from the culture liquid at controlled temps. between 0-50 deg.C by fast removal of the cell material through a slot or opening at a pressure of at least 500 psi but below the pressure which would badly tear the cells. The removed cell material is then stirred in water for 0.1-5 hrs. at 10-50 deg.C to dissolve the enzyme; this is extracted from the inactive cell material in aqs. soln. The cell-free enzymes are used for the prodn. of 6-APA and are esp. suitable for direct removal of side-chains of natural penicillins, esp. the side-chains of benzyl penicillin. The enzymes may be used as starting materials for chemical modification of the enzyme, giving products with higher activity and/or wider technical application.
申请公布号
DE1966427(B2)
申请公布日期
1979.07.12
申请号
DE19691966427
申请日期
1969.02.14
申请人
AB ASTRA, SOEDERTAELJE (SCHWEDEN)
发明人
DELIN, PER STAFFAN;EKSTROEM, BERTIL AKE, DR.;SJOEBERG, BERNDT OLAF HARALD;THELIN, KARL HUGO;NATHORST-WESTFELT, LARS SOELVE, DIPL.-ING.