MONOCLONAL ANTIBODY TO PRION PROTEIN

摘要

PROBLEM TO BE SOLVED: To provide a monoclonal antibody reactive to either of normal prion and abnormal prion derived from different animal species. SOLUTION: This monoclonal antibody is obtained by the following process: for example, prion protein knockout mouse (PrP<0/0> ) is immunized with prion protein, the splenocyte from the thus immunized animal is hybridized with e.g. lymphoblast, and hybridoma cells which acquired HAT resistance in a HAT selective medium are obtained, and from the resulting cell group, the clone in production of the objective antibody meeting the above-mentioned reactivity. For the analysis of the antibody's characteristics, its recognition reactivity to recombinant prion protein and natural prion protein derived from a plurality of animal species is assessed by the use of a combination of immunoprecipitation method with Western blotting method.