| 摘要 |
PROBLEM TO BE SOLVED: To provide compositions, kits, and related methods for the detection and/or monitoring of Salmonella.SOLUTION: Provided are compositions, kits, and methods for the identification of Salmonella. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection. The compositions comprise a T7 provider oligonucleotide and a primer oligonucleotide, wherein the T7 provider oligonucleotide targets a sequence in a region of Salmonella nucleic acid corresponding to bases of about 268-320 of E. coli 23S rRNA, and the primer oligonucleotide targets a sequence in a region of Salmonella nucleic acid, in which the T7 and primer oligonucleotides used in the amplification assay target opposite strands of the Salmonella nucleic acid sequence to be amplified.SELECTED DRAWING: Figure 1 |
