Affinity-controlling material with the use of stimulus-responsive polymer and separation/purification method with the use of the material

摘要

An affinity-controlling material, wherein a stimulus-responsive polymer and an affinitive substance (ligand) having affinity for a target substance are independently attached, preferably covalently, to a support matrix is provided. The material is capable of separating and purifying a target substance such as a physiologically active substance under a physical stimulus while keeping at least one condition other than temperature (for example, pH value of solution, organic solvent concentration or salt concentration) constant by using a support matrix capable of preventing nonspecific adsorption of proteins and achieving an excellent separation performance.