Nano cancer barrier device (NCBD) to immobilize and inhibit the division of metastic cancer stem cells

摘要

The present invention provides methods, compositions, and kits wherein nanomaterials are used for inhibiting cancer stem cell division, colony formation, spheroid formation and self-renewal. The present invention also provides methods, compositions, and kits wherein nanomaterials are used for treating cancer, coating tumors, and inhibiting metastasis. The present invention also provides methods, compositions, and kits wherein nanomaterials are used for inducing cells to go into stasis. The present invention further provides methods for isolating tumors, inhibiting bleeding, and marking margins of tumors and organs during surgery with a nanomaterial.

主权项

1. A method for inducing cell stasis, comprising
(a) contacting the exterior surface of at least one cell with an effective amount of self-assembling peptides to completely surround the cell, wherein the self-assembling peptides have a length of 8 to 200 amino acid residues conforming to one or more of Formulas I-IV:((Xaa+-Xaaneu )x (Xaa−-Xaaneu )y)n,(I)((Xaaneu-Xaa+ )x (Xaaneu-Xaa− )y)n,(II)((Xaaneu-Xaa− )x (Xaaneu-Xaa+)y)n(III)((Xaa−-Xaaneu )x (Xaa+-Xaaneu )y)n, (IV) wherein Xaa+ is an amino acid residue having a positive charge; Xaa− is an amino acid residue having a negative charge; Xaaneu is an amino acid residue having a neutral charge; x and y are integers having a value of 1, 2, or 4, independently; and n is an integer having a value from 1 to 10; and wherein the self-assembling peptides are not substantially self-assembled; and (b) inducing assembly of the self-assembling peptides, to form a self-assembled structure that encapsulates the cell.