DETECTION METHODS EMPLOYING HCV CORE LIPID AND DNA BINDING DOMAIN MONOCLONAL ANTIBODIES

摘要

The present disclosure provides detection methods employing HCV core lipid binding domain and DNA binding domain monoclonal antibodies or antibody fragments. In certain embodiments, the lipid binding domain monoclonal antibody or antibody fragment recognizes an epitope in amino acids 141 to 161 of HCV core protein and the DNA binding domain antibody or antibody fragment recognizes an epitope in amino acids 95-123 (e.g., in amino acids 99-117) of HCV core protein.

主权项

1. A method for the detection of HCV protein in a test sample comprising:
(i) contacting a test sample suspected of containing HCV with a first antibody, or antigen-binding portion thereof, directed against an epitope in the DNA binding domain of an HCV core protein to form a complex between said first antibody, or said antigen-binding portion thereof, and said HCV core protein located within said test sample, wherein said epitope in said DNA binding domain is within amino acids 95-123 of full-length HCV core protein; (ii) contacting said complex formed in step (i) with a second antibody, or antigen-binding portion thereof, directed against an epitope in the lipid binding domain of HCV core protein, to form a complex between said second antibody, or said antigen-binding portion thereof, and said complex formed in step (i), wherein said epitope in said lipid binding domain is within amino acids 141-161 of full-length HCV core protein; and (iii) detecting said complex formed in step (ii).