PHAGE-DISPLAYED ANTIBODY LIBRARIES AND USES THEREOF

摘要

Disclosed herein are phage-displayed single-chain variable fragment (scFv) libraries, which comprised a plurality of scFvs with a specific sequence in each CDR. The present scFv libraries could be used to efficiently produce different antibodies with high binding affinity to H1 hemagglutinin of influenza virus. Accordingly, the present disclosure provides a potential means to generate different antigen-specific antibodies promptly in accordance with the need in experimental researches and/or clinical applications.

主权项

1. A phage-displayed single-chain variable fragment (scFv) library comprising a plurality of scFvs that are expressed by a phage and exhibit binding affinity and specificity to a protein antigen, wherein each of the plurality of scFvs comprises at least one polypeptide selected from the group consisting of,
a first complementarity determining region (CDR) encoded by the nucleotide sequence of SEQ ID NO: 1, a second CDR encoded by the nucleotide sequence of SEQ ID NO: 2, and a third CDR encoded by a the nucleotide sequence of SEQ ID NO: 3.