MODIFIED ALPHA-GLUCOSIDASE AND APPLICATIONS OF SAME

摘要

The object is to provide an α-glucosidase in which a transglycosylation activity predominates, and use thereof, and the like. A modified α-glucosidase consisting of an amino acid sequence in which one or two or more of the amino acid(s) is selected from a group of specific amino acids.

主权项

1. A modified α-glucosidase which (a) consists of an amino acid sequence which is identical to SEQ ID NO: 2 except for a substitution at a position corresponding to position 495 of SEQ ID NO: 2, or (b) comprises an amino acid sequence which has 95% or more identity with SEQ ID NO: 2 and comprises a substitution corresponding to position 495 of SEQ ID NO: 2,
wherein the amino acid at position 495 is substituted by a alanine, glycine, proline, threonine or valine, and wherein the modified α-glucosidase has increased transglycosylation activity to form α-1,6 glucosidic linkages compared to the α-glucosidase of SEQ ID NO: 2.