METHODS FOR NUCLEIC ACID ISOLATION

摘要

This invention relates to improved methods and kits for identification of modified cytosines in a sample nucleotide sequence. Methods comprise treating the nucleic acid sample with bisulfite, adding a suspension of beads in polyethylene glycol without any additional salt such that the nucleic acids bind to the beads, thus enabling purification of the nucleic acid sample. These purification methods may be useful, for example in the analysis of genomic DNA and/or of RNA which has been bisulfite treated.