Recombinant baculovirus vaccine

摘要

The present invention provides a novel transfer vector and a recombinant baculovirus; methods for the production thereof; pharmaceuticals containing the recombinant baculovirus as an active ingredient, which are useful as preventive or therapeutic drugs for infectious diseases such as malaria and influenza; and methods for preventing and treating infectious diseases such as malaria and influenza. More specifically, the invention provides a recombinant transfer vector capable of expressing a foreign gene fused to a virus gene under the control of a dual promoter; a recombinant baculovirus; methods for the production thereof; pharmaceuticals containing the recombinant baculovirus as an active ingredient; and methods for preventing and treating infectious diseases such as malaria and influenza comprising administrating the recombinant baculovirus to patients.

主权项

1. A pharmaceutical composition comprising a recombinant Autographa californica nucleopolyhedrosis virus (AcNPV) as an active agent and, optionally, a pharmaceutically acceptable carrier,
wherein the recombinant AcNPV contains a gene structure having a fusion gene of (A) a gene encoding a partial amino acid sequence of SEQ ID NO:104 (Plasmodium falciparum CircumSporozoite Protein; PfCSP), and (B) a gene encoding a partial amino acid sequence of SEQ ID NO: 114 under the control of a dual promoter comprising a Polyhedrin promoter and a CAG promoter linked to each other (CAP); and wherein the recombinant AcNPV is selected from the group consisting of: (1) a recombinant AcNPV which contains a gene encoding an amino acid sequence consisting of 19-373 amino acid residues of SEQ ID NO:104 as a gene of (A), and a gene encoding an amino acid sequence of SEQ ID NO: 114 as a gene of (B) (AcNPV-CAP-PfCSP), (2) a recombinant AcNPV which contains a gene encoding an amino acid sequence consisting of 19-373 amino acid residues of SEQ ID NO:104 as a gene of (A), and a gene encoding an amino acid sequence consisting of 447-492 amino acid residues of SEQ ID NO: 114 as a gene of (B) (AcNPV-CAP-PfCSP/467), (3) a recombinant AcNPV which contains a gene encoding an amino acid sequence consisting of 205-373 amino acid residues of SEQ ID NO:104 as a gene of (A), and a gene encoding an amino acid sequence of SEQ ID NO: 114 as a gene of (B.) (AcNPV-CAP-CO/205), (4) a recombinant AcNPV which contains a gene encoding an amino acid sequence consisting of 76-373 amino acid residues of SEQ ID NO:104 as a gene of (A), and a gene encoding an amino acid sequence consisting of 447-492 amino acid residues of SEQ ID NO: 114 as a gene of (B) (AcNPV-CAP-CO/76/467), (5) a recombinant AcNPV which contains a gene encoding an amino acid sequence consisting of 76-373 amino acid residues of SEQ ID NO:104 as a gene of (A), and a gene encoding an amino acid sequence of SEQ ID NO: 114 as a gene of (B) (AcNPV-CAP-CO/76), (6) a recombinant AcNPV which contains a gene encoding an amino acid sequence consisting of 1-397 amino acid residues of SEQ ID NO:104 as a gene of (A), and a gene encoding an amino acid sequence of SEQ ID NO: 114 as a gene of (B) (AcNPV-CAP-CO/full).