Screening method utilizing thalidomide-targeting factor

摘要

With an aim to provide means for developing a compound devoid of teratogenicity but retaining beneficial actions, a screening method for a non-teratogenic substance comprising bringing a test substance into contact with cereblon or a fragment of cereblon, evaluating the bindability of the test substance with cereblon or the fragment of cereblon, and selecting a test substance that does not bind to cereblon or the fragment of cereblon or a test substance exhibiting lower bindability with cereblon or the fragment of cereblon than does thalidomide is provided.

主权项

1. A screening method for a substance that does not have thalidomide-like teratogenicity comprising:
bringing a test substance into contact with cereblon or a fragment of cereblon, and selecting a test substance that does not bind to cereblon or the fragment of cereblon or a test substance which has a lower affinity for cereblon as compared with thalidomide under similar conditions, wherein cereblon or the fragment of cereblon have a sequence selected from the group of sequences consisting of SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15 and SEQ ID NO:16, and wherein when the test substance does not bind to cereblon or the fragment of cereblon, or has lower affinity for cereblon or the fragment of cereblon as compared with thalidomide, the test substance is determined to not have thalidomide-like teratogenicity.