发明名称 |
INTEIN MEDIATED PURIFICATION OF PROTEIN |
摘要 |
The present invention includes methods, compositions, uses, and kits for purifying a protein of interest (POI) comprising contacting a first fusion protein comprising the POI fused to the C-terminus of an intein C-fragment with a second fusion protein comprising an intein N-fragment and a purification tag to form a complex between the first fusion protein and the second fusion protein, cleaving the POI from the intein C-fragment, wherein the protein is released from the complex; and isolating the POI; the present invention also includes fusion proteins and vector. |
申请公布号 |
EP2943573(A1) |
申请公布日期 |
2015.11.18 |
申请号 |
EP20140702146 |
申请日期 |
2014.01.10 |
申请人 |
THE TEXAS A&M UNIVERSITY SYSTEM |
发明人 |
CHEN, ZHILEI;RAMIREZ, MIGUEL, A.;GUAN, DONGLI |
分类号 |
C12N15/62;C07K1/32 |
主分类号 |
C12N15/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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