Recombinant mycobacterium avium subsp. paratuberculosis proteins induce immunity and protect against infection

摘要

Compositions of immunogenic proteins of Mycobacterium avium subspecies paratuberculosis (MAP) are effective for stimulating a protective immune response in animals against MAP. Combinations of MAP1087 with two or more of MAP1204, MAP1272c and MAP2077c are effective for stimulating a protective immune response in animals against MAP and can be administered as vaccines against paratuberculosis (Johne's disease). Induction of the immune response significantly reduces or eliminates colonization of the animal by MAP, and consequently reduces or eliminates the symptoms of clinical disease in animals infected with MAP and reduces or eliminates fecal shedding of MAP. Vaccination with the compositions provides protection against clinical disease and reduces transmission of MAP infection within a herd.

主权项

1. A composition comprising isolated and purified immunogenic proteins of Mycobacterium avium subspecies paratuberculosis and a pharmaceutically acceptable carrier, wherein said immunogenic proteins are provided in an amount effective to stimulate an immune response in an animal against said Mycobacterium avium subspecies paratuberculosis, and said immunogenic proteins comprise MAP1087 and at least two of MAP1204, MAP1272c, and MAP2077c wherein said MAP1087 comprises SEQ ID NO: 1; wherein said MAP1204 comprises SEQ ID NO: 2; wherein said MAP1272c comprises SEQ ID NO: 3; and wherein said MAP2077c comprises SEQ ID NO: 4, wherein said pharmaceutically acceptable carrier is selected from the group consisting of physiological saline, phosphate buffered saline, mineral oil, vegetable oil, aqueous carboxymethyl cellulose, and polyvinylpyrrolidone.