| 摘要 |
<p>The invention relates to a method for recovering a specific cellular component from a microorganism, which comprises steps of: providing a biomass of a microorganism, adding algal cytotoxin or a chemical analogue or derivative thereof, which is capable of rupturing the cell wall and / or cell membrane of the microorganism and also cell wall and/or cell membrane of at least one of the following:
microalgae selected from the group consisting of species of the genera Achnantes, Agmenellum,Amphiprora, Amphora, Anabaena, Anabaenopsis, Ankistrodesmus, Arthrospira, Attheya, Boeklovia, Botryococcus, Biddulphia, Brachiomonas, Bracteococcus, Carteria, Chaetoceros, Characium, Chlamydomonas, Cricophaera, Crypthecodinium, Cryptomonas, Chlorella, Chlorococcum, Chrysophaera, Coccochloris, Cocconeis, Cyclotella, Cylindrotheca, Dermocarpa, Dunaliella, Ellipsoidon, Entomoneis, Euglena, Eremosphaera, Extubocellulus, Franceia, Fragilaria, Gleocapsa, Gleothamnion, Hantzschia, Haematococcus, Hormotilopsis, Hymenomonas, Isochrysis, Lepocinclis, Melosira, Minidiscus, Micractinum, Microcystis, Monallanthus, Monoraphidium, Muriellopsis, Nannochloris, Nannochloropsis, Navicula, Neochloris, Nephroselmis, Nitzschia, Nodularia, Nostoc, Ochromonas, Oedogonium, Oocystis, Oscillatoria, Papiliocellulus, Parachlorella, Pascheria, Pavlova, Peridinium, Phaeodactylum, Phagus, Plankthothrix, Platymonas, Plectonema, Pleurochrysis, Phormidium, Pleurosigma, Porphyridium, Prymnesium, Pseudochlorella, Pyramimonas, Pyrobotrus,Radiosphaera, Rhodomonas, Rhodosorus, Sarcinoid, Scenedesmus, Schizochytrium, Scrippsiella, Seminavis, Skeletonema, Spirogyra, Spirulina, Stichococcus, Synechococcus, Synechocystis, Synedra, Tetraedron, Tetraselmis, Thalassiosira, Trachyneis, Traustrochytrium, Trentepholia, Ulkenia, Viridiella, Volvox and Xenococcus
or bacteria selected from the group consisting of the genera Acinetobacter, Actinobacter, Aerogenes, Alcanivorax, Arthrobacter, Bacillus, Clostridium, Cupriviadus, Dietzia, Gordonia, Escherichia, Flexibacterium, Micrococcus, Mycobacterium, Nocardia, Pseudomonas, Ralstonia, Rhodococcus, Rhodomicrobium, Rhodopseudomonas, Shewanella, Shigella, Streptomyces, Wautersia and Vibrio or yeasts selected from the group consisting of the genera Candida, Clavispora, Cryptococcus, Deparyomyces, Endomycopsis, Galactomyces, Hansenula, Kluyveromyces, Lipomyces, Pachysolen, Pichia, Rhodosporidium, Rhodotorula, Saccharomyces, Waltomyces, Yarrowia and Trichosporon or
filamentous fungi selected from the group consisting of the genera Absidia, Aspergillus, Blakeslea, Chaetomium, Cladosporium, Claviceps, Cladosporidium, Cunninghamella, Emericella, Entomophthora, Fusarium, Gibberella, Glomus, Humicola, Malbranchea, Mucor, Mortierella, Paecilomyces, Penicillium, Puccia, Pythium, Rhizopus, Saprolegnia, Trichoderma, Thermomycs, Ustilago and Zygorhynchus to the microbial biomass,
-releasing an intracellular component from the cell, and
-recovering said intracellular component. The invention relates also a composition used for rupturing lipid-producing algae cells and an integrated system for rupturing lipid-producing algae cells.</p> |
