METHOD FOR DETECTION OF DRUG USING LIQUID-LIQUID EXTRACTION BY MIXED ETHYL ACETATE AND ACETONITRILE

摘要

The present invention relates to a method for detecting a drug comprising a liquid-liquid extraction by using an organic solvent mixed solution of ethyl acetate, which is a non-polar organic solvent, and acetonitrile, which is an anodic organic solvent. The method has a higher efficiency of extracting drugs than existing liquid-liquid extraction methods that only use ethyl acetate, thereby enabling a more effective detection of various drugs. [Reference numerals] (AA) (First step) Step of mixing an organic solvent mixed solution of ethyl acetate and acetonitrile in a diluted solution of a drug standard product and performing a liquid-liquid extraction; (BB) (Second step) Step of conducting centrifugation for the liquid-liquid extracted mixed solution in the first step and obtaining a supernatant as an organic solvent layer; (CC) (Third step) Step of drying the supernatant separated in the second step using nitrogen gas and dissolving the dried sample again into a solvent in mobile phase for chromatography; (DD) (Fourth step) Step of quantitatively analyzing the sample dissolved into the solvent in mobile phase of the third step using the chromatography