发明名称 |
Reporter gene assay method |
摘要 |
The invention provides a reporter gene assay method by which the sex hormone-like activity inherent in a test substance can be accurately assayed excluding the effects caused by a decrease in cell activity (protein expressing capacity). The assay method is a reporter gene assay method using luciferase-expressing cells which comprises further transferring a GFP gene into the luciferase-expressing cells, measuring the luciferase expression level and GFP expression level, and making a judgment about the thus-measured luciferase expression level using the decrease in GFP expression level as an indication of the decrease in cell activity. |
申请公布号 |
US8609342(B2) |
申请公布日期 |
2013.12.17 |
申请号 |
US20020498265 |
申请日期 |
2002.12.13 |
申请人 |
IIDA MITSURU;OGURI HIDEO;OTSUKA PHARMACEUTICAL CO., LTD. |
发明人 |
IIDA MITSURU;OGURI HIDEO |
分类号 |
C12Q1/68;G01N33/483;C12N5/10;C12N15/09;C12Q1/02;C12Q1/66;G01N21/78;G01N33/00;G01N33/74 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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