发明名称 |
MEMBRANE-TRANSLOCATING PEPTIDES |
摘要 |
A method is provided for selecting membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalised nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.
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申请公布号 |
CA2658491(C) |
申请公布日期 |
2013.09.24 |
申请号 |
CA20062658491 |
申请日期 |
2006.07.24 |
申请人 |
ISOGENICA LTD |
发明人 |
COOMBER, DAVID;FITZGERALD, KEVIN;MCGREGOR, DUNCAN;ULLMAN, CHRIS;LEANDERSON, TOMAS |
分类号 |
C40B20/04;A61K9/127;A61K47/48;C07K1/00;C07K5/00;C07K7/00;C12N15/00;C12N15/09;C12N15/10;C12Q1/68;C40B30/00;C40B50/06;G01N33/68 |
主分类号 |
C40B20/04 |
代理机构 |
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代理人 |
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地址 |
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