发明名称 |
Optimized DNA sequence of an antibody to improve quality and yield of bacterially expressed antibody fusion proteins |
摘要 |
Object matter of the invention is an optimized DNA sequence encoding the scFv(FRP5) antibody fragment. This novel sequence prevents the generation of the undesired by-product in the context of an scFv(FRP5)-ETA fusion protein, and possibly also other bacterially expressed scFv(FRP5)-containing fusion proteins. The DNA sequence of the scFv(FRP5) domain of scFv(FRP5)-ETA was modified by exchanging a distinct codon, thereby preventing an otherwise possible internal start of protein translation.
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申请公布号 |
US8530637(B2) |
申请公布日期 |
2013.09.10 |
申请号 |
US20110985542 |
申请日期 |
2011.01.06 |
申请人 |
WELS WINFRIED S.;DAELKEN BENJAMIN;SCHWARZ SYLVIA E.;CHEMOTHERAPEUTISCHES FORSCHUNGSINSTITUT GEORG-SPEYER-HAUS |
发明人 |
WELS WINFRIED S.;DAELKEN BENJAMIN;SCHWARZ SYLVIA E. |
分类号 |
C07H21/04 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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