发明名称 |
Vectors with modified initiation codon for the translation of AAV-Rep78 useful for production of AAV |
摘要 |
The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
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申请公布号 |
US8512981(B2) |
申请公布日期 |
2013.08.20 |
申请号 |
US20070306239 |
申请日期 |
2007.06.20 |
申请人 |
HERMENS WILHELMUS THEODORUS JOHANNES MARIA CHRISTIAAN;HAAST SASKIA JACOBA PETRONELLA;BIESMANS DENNIS JOHAN;BAKKER ANDREW CHRISTIAN;AMSTERDAM MOLECULAR THERAPEUTICS B.V. |
发明人 |
HERMENS WILHELMUS THEODORUS JOHANNES MARIA CHRISTIAAN;HAAST SASKIA JACOBA PETRONELLA;BIESMANS DENNIS JOHAN;BAKKER ANDREW CHRISTIAN |
分类号 |
C12N5/02;C12N7/00;C12N7/01;C12N7/02 |
主分类号 |
C12N5/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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