发明名称 |
METHOD FOR GENERATING STABLE CELL LINES EXPRESSING HIGH LEVELS OF A PROTEIN OF INTEREST |
摘要 |
This invention relates to industrial production of proteins. More specifically, the invention relates to a method for obtaining cells that stably express a protein of interest, even when cultivated in the absence of selective pressure. DHFR is used as a surrogate marker. The transfected cells are not selected based on resistance to a toxic compound, but based on fluorescence as measured by FACS using fluorescent MTX. |
申请公布号 |
EA017398(B1) |
申请公布日期 |
2012.12.28 |
申请号 |
EA20100070152 |
申请日期 |
2008.07.16 |
申请人 |
MERCK SERONO S.A. |
发明人 |
CRAWFORD MC FADD TARA, KRISTEN;YANG MEIJIA |
分类号 |
C12N15/65;C12N15/67;C12N15/79 |
主分类号 |
C12N15/65 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|