| 摘要 |
An endoglucanase gene or a ß-glucosidase gene can be identified by isolating genomic DNA containing a gene for a cellulase that is classified as an endoglucanase or a ß-glucosidase from Acremonium cellulolyticus and analyzing the nucleotide sequence for the genomic DNA. Amino acid sequences for known endoglucanases and ß-glucosidases are compared with each other extensively, and an amino acid sequence which can be conserved in Acremonium cellulolyticus is found. Based on the information for the amino acid sequence, various primers are designed. Using the various primers thus designed, a PCR is carried out employing genomic DNA or cDNA as a template. As a result, fragments of genes for endoglucanases and genes for ß-glucosidases can be obtained. Primers are designed based on the gene fragments, the PCR is continued using the primers to amplify each of a total of nine endoglucanase and ß-glucosidase genes, and the nucleotide sequences for the amplification products are analyzed. |
